10107921001
Roche
Nuclease S7
Micrococcal nuclease, from Staphylococcus aureus
Sinónimos:
s7 nuclease
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About This Item
Código UNSPSC:
12352204
Productos recomendados
origen biológico
Staphylococcus aureus
Nivel de calidad
Formulario
lyophilized
actividad específica
~15,000 U/mg
envase
pkg of 15,000 U
fabricante / nombre comercial
Roche
pH óptimo
7.5
Condiciones de envío
wet ice
Aplicación
- Removal of endogenous RNA from in vitro translation systems
- RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.
Acciones bioquímicas o fisiológicas
Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
Definición de unidad
One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.
Almacenamiento y estabilidad
Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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