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Key Documents

OP187

Sigma-Aldrich

Anti-EpCAM-1 (Ab-1) Mouse mAb (VU-1D9)

liquid, clone VU-1D9, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

VU-1D9, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPCAM(4072)

General description

Anti-EpCAM-1 (Ab-1), mouse monoclonal, clone VU-1D9, recognizes the ~40 kDa EpCAM-1 in squamous cell carcinoma. It is validated for use in WB, FC, IP, and IHC (frozen and paraffin sections).
Protein A purified mouse monoclonal antibody. Recognizes the ~40 kDa EpCAM-1 protein.
Recognizes the ~40 kDa EpCAM-1 protein in squamous cell carcinoma.

Immunogen

Human
NCI-H69 human small cell lung carcinoma cells

Application

Flow Cytometry (1-3 µg/ml)

Frozen Sections (1-3 µg/ml)

Immunoblotting (1-3 µg/ml)

Immunoprecipitation (1-3 µg/ml)

Paraffin Sections (1-3 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C)

Analysis Note

Positive Control
Squamous cell carcinoma

Other Notes

Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mathilde Dusséaux et al.
Gastroenterology, 153(6), 1647-1661 (2017-08-31)
Hepatitis B virus (HBV) infects hepatocytes, but the mechanisms of the immune response against the virus and how it affects disease progression are unclear. We performed studies with BALB/c Rag2-/-Il2rg-/-SirpaNODAlb-uPAtg/tg mice, stably engrafted with human hepatocytes (HUHEP) with or without
Farhad Khosravi et al.
Nanotechnology, 27(13), 13LT02-13LT02 (2016-02-24)
We demonstrate the rapid and label-free capture of breast cancer cells spiked in buffy coats using nanotube-antibody micro-arrays. Single wall carbon nanotube arrays were manufactured using photo-lithography, metal deposition, and etching techniques. Anti-epithelial cell adhesion molecule (EpCAM) antibodies were functionalized
Bang Wool Eom et al.
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 32(5), 614-620 (2020-11-24)
There has been a demand for a tumor-specific marker for metastatic lymph nodes in sentinel navigation surgery for gastric cancer. The aim of this study is to analyze protein expression in both primary tumors and metastatic lymph nodes in early
Farhad Khosravi et al.
Nanotechnology, 27(44), 44LT03-44LT03 (2016-09-30)
We demonstrate the rapid and label-free capture of breast cancer cells spiked in blood using nanotube-antibody micro-arrays. 76-element single wall carbon nanotube arrays were manufactured using photo-lithography, metal deposition, and etching techniques. Anti-epithelial cell adhesion molecule (anti-EpCAM), Anti-human epithelial growth

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