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NI03

Sigma-Aldrich

Normal Mouse IgG

This Normal Mouse IgG is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Mouse IgG, Non-immune.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

serum

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

General description

Native IgG from mouse serum. IgG is a glycoprotein found in serum and tissue fluids. For use as a negative control in parallel with specific mouse IgG-type primary antibodies.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Other Notes

Normal IgG are provided to be used as negative controls for purified antibodies from Calbiochem. For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Lei Gao et al.
Nature communications, 10(1), 1665-1665 (2019-04-12)
Lung cancer is the leading cause of cancer-related deaths worldwide. Tumor suppressor genes remain to be systemically identified for lung cancer. Through the genome-wide screening of tumor-suppressive transcription factors, we demonstrate here that GATA4 functions as an essential tumor suppressor
Sandhya Yadav et al.
BMC cancer, 19(1), 1031-1031 (2019-11-05)
The deregulated alternative splicing of key glycolytic enzyme, Pyruvate Kinase muscle isoenzyme (PKM) is implicated in metabolic adaptation of cancer cells. The splicing switch from normal PKM1 to cancer-specific PKM2 isoform allows the cancer cells to meet their energy and
Chuan Li et al.
Epigenetics & chromatin, 12(1), 23-23 (2019-04-18)
Transcription from the integrated HIV-1 promoter is directly governed by its chromatin environment, and the nucleosome-1 downstream from the transcription start site directly impedes transcription from the HIV-1 promoter. The HIV-1 Tat protein regulates the passage from viral latency to

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