MABS87
Anti-GPR177 Antibody, clone YJ5
clone YJ5, from mouse
Sinónimos:
Protein wntless homolog, Integral membrane protein GPR177, Protein evenness interrupted homolog, Putative NF-kappa-B-activating protein 373, WLS, EVI
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
YJ5, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WLS(79971)
General description
Gpr177 (WLS, EVI), the mouse ortholog of Drosophila WIs, is expressed during mouse embryogenesis. It is essential for the patterning of the anterior-posterior axis during mammalian development and is activated by â-catenin and LEF/TCF-dependent transcription. Upon GPR177 activation, Wnt binds with and modifies the cellular distribution of GPR177, leading to a feedback regulatory mechanism involving Wnt expression and signaling.
Immunogen
Histidine-tagged recombinant protein corresponding to human GPR177.
Application
Anti-GPR177 Antibody, clone YJ5 is an antibody against GPR177 for use in WB & IC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GPR177 in HeLa cells (Prof. D.M. Virshup, Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School.) (Also reference Coombs, G.S., et al. (2010). J Cell Sci. 123(19):3357-3367).
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.
Target description
~62 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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