MABS443

Anti-GRP78 Antibody, clone C38

clone C38, from mouse

• Sinónimos: 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: C38, monoclonal
• reactividad de especies: human
• técnicas: flow cytometry: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_005338
• Nº de acceso UniProt: P11021
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HSPA5(3309)

Descripción

Descripción general

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.

Inmunógeno

Recombinant protein corresponding to human GRP78.

Aplicación

Anti-GRP78 Antibody, clone C38 detects level of GRP78 & has been published & validated for use in GRP78.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected GRP78 in HepG2 cells (de Ridder, G. G., et al. (2012). Melanoma Res. 22(3):225-235.).

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Calidad

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected GRP78 in 1X10E6 HeLa cells.

Descripción de destino

72 kDa calculated

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable