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MAB5316

Sigma-Aldrich

Anti-Rhodopsin Antibody, clone RET-P1

clone RET-P1, Chemicon®, from mouse

Sinónimos:

Opsin 2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

RET-P1, monoclonal

reactividad de especies

human, mouse, rat

fabricante / nombre comercial

Chemicon®

técnicas

immunocytochemistry: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... RHO(6010)

Especificidad

Reacts with a protein of 39 kDa identified as rhodopsin (opsin). MAB5316 specifically labels the axons and synaptic pedicles of the rods.

CELLULAR LOCALIZATION:

Cytoplasmic

Inmunógeno

Membrane preparation from adult rat retina.

Aplicación

Detect Rhodopsin using this Anti-Rhodopsin Antibody, clone RET-P1 validated for use in IC & WB.
Immunoblotting: 1 μg/mL

Immunocytochemistry

Immunohistochemistry (frozen and formalin/paraffin): 1-2 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS

Forma física

Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Nota de análisis

Control
POSITIVE CONTROL:

IMR-5 cells, brain or retina.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Structural and functional effects of hemiretinal endodiathermy axotomy in cynomolgus macaques.
Dashek, RJ; Kim, CB; Rasmussen, CA; Hennes-Beean, EA; Ver Hoeve, JN; Nork, TM
Investigative Ophthalmology & Visual Science null
Ayse Sahaboglu et al.
PloS one, 12(7), e0181374-e0181374 (2017-07-21)
Peripherin (peripherin/rds) is a membrane-associated protein that plays a critical role in the morphogenesis of rod and cone photoreceptor outer segments. Mutations in the corresponding PRPH2 gene cause different types of retinal dystrophies characterized by a loss of photoreceptors. Over
Birgit Ploier et al.
Nature communications, 7, 12832-12832 (2016-10-04)
Retinitis pigmentosa (RP) is a blinding disease often associated with mutations in rhodopsin, a light-sensing G protein-coupled receptor and phospholipid scramblase. Most RP-associated mutations affect rhodopsin's activity or transport to disc membranes. Intriguingly, some mutations produce apparently normal rhodopsins that
Steroids do not prevent photoreceptor degeneration in the light-exposed T4R rhodopsin mutant dog retina irrespective of AP-1 inhibition.
Gu, D; Beltran, WA; Pearce-Kelling, S; Li, Z; Acland, GM; Aguirre, GD
Investigative Ophthalmology & Visual Science null
Astrid Zayas-Santiago et al.
Molecular vision, 15, 1-9 (2009-01-13)
Intact adult photoreceptors in culture can be a valuable tool in the search of therapies for retinal degenerations. The major challenge in this technique is that photoreceptors undergo an alteration in cytoarchitecture and loss of outer segment during the cell

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