MAB2030
Anti-CSPG4 Antibody
CHEMICON®, mouse monoclonal, BE-123
Sinónimos:
Anti-CSPG4A, Anti-HMW-MAA, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
Nombre del producto
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123, ascites fluid, clone BE-123, Chemicon®
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
ascites fluid
tipo de anticuerpo
primary antibodies
clon
BE-123, monoclonal
reactividad de especies
human, canine, rat, sheep, bovine, mouse
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
radioimmunoassay: suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CSPG4(1464)
Especificidad
Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.
Inmunógeno
Mouse proteoglycan.
Aplicación
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
ELISA at 1:400-1:3,200.
RIA at 1:50-1:6,400.
Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)
Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.
Optimal working dilutions must be determined by the end user.
RIA at 1:50-1:6,400.
Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)
Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Forma física
Ascites fluid containing no preservatives.
Almacenamiento y estabilidad
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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