MAB10754

Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1

clone 2B12.1, from mouse

• Sinónimos: caspase 8, apoptosis-related cysteine peptidase, caspase-8, caspase 8, apoptosis-related cysteine protease, MACH-beta-1/2/3/4 protein, Apoptotic protease Mch-5, Apoptotic cysteine protease, MACH-alpha-1/2/3 protein, FADD-homologous ICE/ced-3-like proteas
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 2B12.1, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgMκ
• Nº de acceso NCBI: NP_001073593
• Nº de acceso UniProt: Q14790
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CASP8(841)

Descripción

Descripción general

Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death. Caspase-8 is recruited to death-inducing signaling complex (DISC), CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18. Active caspase-8 or the p18 subunit, is the first in the caspase signaling cascade, and it signals downstream caspases to eventually induce apoptosis.

Especificidad

This antibody recognizes the active form (p18 subunit) of Caspase-8.

Inmunógeno

KLH-conjugated linear peptide corresponding to the active form of human Caspase-8.

Aplicación

Detect Caspase-8 (active form p18 subunit) using this Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1 validated for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected active Caspase-8 (p18 subunit) in etoposide-treated HeLa cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Caspases

Apoptosis - Additional

Calidad

Evaluated by Western Blot in camptothecin-treated and untreated Jurkat lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected the active form of Caspase-8 (p18 subunit) in 10 µg of camptothecin-treated Jurkat lysates. Untreated jurkat lysate exhibited no detection of active Caspase-8.

Descripción de destino

~18 kDa observed

Ligadura / enlace

Replaces: 04-573; 04-574

Forma física

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Camptothecin-treated and untreated Jurkat lysates

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable