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ABC11

Sigma-Aldrich

Anti-Bax (NT) Antibody

from rabbit

Sinónimos:

BCL2-associated X protein, Bcl-2-like protein 4, apoptosis regulator BAX

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), feline (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_620119

UniProt accession no.

Q07812

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BAX(581)

General description

BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
The previously assigned protein identifier Q07815 has been merged into Q07812. Full details can be found on the UniProt database.

Specificity

Rat (90%), Mouse (86%).
This antibody recognizes BAX.

Immunogen

KLH-conjugated linear peptide corresponding to human BAX at and around the N-terminus.

Application

Anti-Bax Antibody is an antibody against Bax (N-terminus) for use in WB, IP, IC, IH.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated BAX from 500 µg of HL60 cell lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected BAX in colorectal and ductal carcinoma tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BAX in MCF7 cells.

Quality

Evaluated by Western Blot in HL60 cell lysate.

Western Blot Analysis: 0.125 µg/ml of this antibody detected BAX on 10 µg of HL60 cell lysate.

Target description

~ 21 kDa

Linkage

Replaces: 06-499

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Dan-Dan Sun et al.
Journal of dental sciences, 18(1), 310-321 (2023-01-17)
Periodontitis is a prevalent infectious inflammatory disease. Growing evidence has revealed important roles for circular RNAs (circRNAs) and circRNA sponge activity in periodontitis. Here, we elucidated the precise part of circ_0097010 in periodontitis pathogenesis. Human periodontal ligament cells (hPDLCs) were
Teruyoshi Saito et al.
Oncology reports, 29(1), 335-342 (2012-11-06)
Hyaluronan (HA), a major component of the extracellular matrix (ECM), is synthesized by HA synthase (HAS) 1, HAS2 and HAS3 and is intricately involved
Adeline Y Robin et al.
Structure (London, England : 1993), 26(10), 1346-1359 (2018-08-21)
BAX and BAK are essential mediators of intrinsic apoptosis that permeabilize the mitochondrial outer membrane. BAX activation requires its translocation from cytosol to mitochondria where conformational changes cause its oligomerization. To better understand the critical step of translocation, we examined
Sweta Iyer et al.
Nature communications, 7, 11734-11734 (2016-05-25)
During apoptosis, Bak and Bax are activated by BH3-only proteins binding to the α2-α5 hydrophobic groove; Bax is also activated via a rear pocket. Here we report that antibodies can directly activate Bak and mitochondrial Bax by binding to the
Bo Zhou et al.
Cell research, 28(12), 1171-1185 (2018-10-06)
Iron has been shown to trigger oxidative stress by elevating reactive oxygen species (ROS) and to participate in different modes of cell death, such as ferroptosis, apoptosis and necroptosis. However, whether iron-elevated ROS is also linked to pyroptosis has not
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