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Key Documents

AB3852

Sigma-Aldrich

Anti-Cortactin Antibody, phospho-specific (Tyr421)

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr421)

Gene Information

human ... CTTN(2017)

Specificity

Recognizes Cortactin phosphorylated on tyrosine 421. Cortactin is an 80-85 kDa actin-binding protein, which consists of a variety of protein domains. The nature and function of these domains, coupled with the presence of multiple consensus sites for phosphorylation by tyrosine and serine/threonine kinases, suggests that cortactin integrates activation of specific signaling pathways with cytoskeletal reorganization.

Immunogen

Epitope: phospho-specific (Tyr421)
Rabbit anti-Cortactin (Y421) polyclonal antibody was raised against a peptide corresponding to residues surrounding phosphorylated Y421of human cortactin.

Application

Anti-Cortactin Antibody, phospho-specific (Tyr421) detects level of Cortactin & has been published & validated for use in WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blot: 1-2 μg/mL

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in 1mg/mL in PBS with 0.05% Bronidox.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Hippocampal Cortactin Levels are Reduced Following Spatial Working Memory Formation, an Effect Blocked by Chronic Calpain Inhibition.
Olson, ML; Ingebretson, AE; Harmelink, KM
Brain sciences null
Nayden G Naydenov et al.
Cells, 11(9) (2022-05-15)
Recurrent chronic mucosal inflammation, a characteristic of inflammatory bowel diseases (IBD), perturbs the intestinal epithelial homeostasis resulting in formation of mucosal wounds and, in most severe cases, leads to colitis-associated colon cancer (CAC). The altered structure of epithelial cell-cell adhesions
Jakob Knorr et al.
European journal of microbiology & immunology, 10(1), 20-27 (2020-05-05)
The gastric pathogen Helicobacter pylori colonizes approximately half of the human world population. The bacterium injects the effector protein cytotoxin associated gene A (CagA) via a type-IV secretion system into host epithelial cells, where the protein becomes phosphorylated at specific
Alexis Bonfim-Melo et al.
Cellular microbiology, 17(12), 1797-1810 (2015-06-23)
Trypanosoma cruzi extracellular amastigotes (EAs) display unique mechanisms for cell invasion that are highly dependent on host actin filaments. Protein kinase D1 (PKD1) phosphorylates and modulates the activity of cortactin, a key regulator of actin dynamics. We evaluated the role
Yu Yu et al.
Oncogene, 37(28), 3778-3789 (2018-04-13)
Cell motility and invasiveness are prerequisites for dissemination, and largely account for cancer mortality. We have identified an actionable kinase, spleen tyrosine kinase (SYK), which is keenly tightly associated with tumor progression in ovarian cancer. Here, we report that active

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