AB2281

Anti-Na+K+Cl- Cotransporter 2 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter, NKCC2A variant A, Na-K-2Cl cotransporter, sodium potassium chloride cotransporter 2, solute carrier family 12 (sodium/potassium/chloride transporters), m
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, rat, human
• técnicas: western blot: suitable
• Nº de acceso NCBI: NM_000338.2
• Nº de acceso UniProt: Q13621
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SLC12A1(6557)

Descripción

Descripción general

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle′s loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. SLC12A1 is kidney specific and is essential in the regulation of ionic balance and cell volume. It is a component of an electrically silent transporter system, mediating sodium and chloride reabsorption. Defects in SLC12A1 are the cause of Bartter syndrome type 1, an autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria.

Especificidad

This antibody recognizes the cytoplasmic domain of NA+K+CL- Cotransporter 2.

Inmunógeno

Epitope: Cytoplasmic domain

KLH conjugate followed by NA+K+CL- Cotransporter 2 corresponding to the cytoplasmic domain.

Aplicación

Anti-Na+K+Cl-Cotransporter 2 Antibody detects level of Na+K+Cl-Cotransporter 2 & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Calidad

Western Blot Analysis:
1:2,000 dilution of this lot detected NA+K+CL- Cotransporter 2 on 10 µg of rat liver lysate.

Descripción de destino

~150 kDa

Forma física

Antigen Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Almacenamiento y estabilidad

Stable for 1 year at 2-8ºC from date of receipt.

Nota de análisis

Control
Rat liver lysates

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable