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Merck
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Key Documents

AB1586

Sigma-Aldrich

Anti-Ubiquitin Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, sheep, eukaryotes, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBAP2(55833)

Specificity

Recognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.

Immunogen

Ubiquitin from bovine red blood cells coupled to chicken gamma globulin.

Application

Detect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH.
Immunohistochemistry: 1:2,000-1:5,000.

Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).

Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Linkage

Replaces: 04-454

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Xiao-Wen Lin et al.
PloS one, 8(6), e67633-e67633 (2013-06-27)
Toll-like receptors (TLRs) play a pivotal role in the defense against invading pathogens by detecting pathogen-associated molecular patterns (PAMPs). TLR4 recognizes lipopolysaccharides (LPS) in the cell walls of Gram-negative bacteria, resulting in the induction and secretion of proinflammatory cytokines such

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