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AB15858

Sigma-Aldrich

Anti-Aquaporin 5 Antibody

1 mg/mL, Chemicon®

Sinónimos:

AQP5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_036911

UniProt accession no.

P47864

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Aqp5(11830)
rat ... Aqp5(25241)

General description

Aquaporins (AQPs) are a family of channel proteins that allow water or very small solutes to pass, functioning in tissues where the rapid and regulated transport of fluid is necessary, such as the kidney, lung, and salivary glands. Aquaporin 5 is considered to play an important role in water movement across the plasma membrane in salivary fluid secretion and corneal/lens lubrication and is a focus of studies illucidating bechanisms underlying diabetic xerostomia.

Specificity

Aquaporin 5 (AQP5). By Western blot the antibody reacts with a band at ~ 24 kDa on rat submaxillary gland lysate.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat Aquaporin 5.

Application

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Aquaporin 5 in rat lung and mouse trachea tissue.
Immunohistochemistry Analysis: A representative lot from independent laboratories detected Aquaporin 5 in various paraformaldehyde fixed tissues (see references).
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-Aquaporin 5 (AQP5) Antibody is validated for use in WB for the detection of Aquaporin 5 (AQP5).

Quality

Evaluated by Western Blotting in rat salivary gland tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Aquaporin 5 in 10 µg of rat salivary gland tissue lysate.

Target description

~28 kDa observed

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analysis Note

Control
Tested on Rat Salivary Gland Lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Yosuke Nakazawa et al.
Cells, 10(2) (2021-03-07)
Presbyopia is characterized by a decline in the ability to accommodate the lens. The most commonly accepted theory for the onset of presbyopia is an age-related increase in the stiffness of the lens. However, the cause of lens sclerosis remains
Verification and spatial localization of aquaporin-5 in the ocular lens.
Grey, AC; Walker, KL; Petrova, RS; Han, J; Wilmarth, PA; David, LL; Donaldson, PJ; Schey, KL
Experimental Eye Research null
Yao Chen et al.
Respiratory research, 13, 1-1 (2012-01-05)
Distal alveolar morphogenesis is marked by differentiation of alveolar type (AT)-II to AT-I cells that give rise to the primary site of gas exchange, the alveolar/vascular interface. Endothelial-Monocyte Activating Polypeptide (EMAP) II, an endogenous protein with anti-angiogenic properties, profoundly disrupts
Rosica S Petrova et al.
American journal of physiology. Cell physiology, 314(2), C191-C201 (2017-11-10)
Although the functionality of the lens water channels aquaporin 1 (AQP1; epithelium) and AQP0 (fiber cells) is well established, less is known about the role of AQP5 in the lens. Since in other tissues AQP5 functions as a regulated water
Romell B Gletten et al.
Investigative ophthalmology & visual science, 63(8), 5-5 (2022-07-12)
To spatially map aquaporin-5 (AQP5) expression in the bovine lens, molecularly characterize cytoplasmic AQP5-containing vesicles in the outer cortex, and elucidate AQP5 membrane trafficking mechanisms. Immunofluorescence was performed on bovine lens cryosections using AQP5, TOMM20, COX IV, calnexin, LC3B, Sec22β
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