324414

Dynamin Inhibitor V, 34-2

The Dynamin Inhibitor V, 34-2 controls the biological activity of Dynamin. This small molecule/inhibitor is primarily used for Membrane applications.

• Sinónimos: Dynamin Inhibitor V, 34-2, 2-Cyano-N-octyl-3-(1-(3-dimethylaminopropyl)-1H-indol-3-yl)-acrylamide
• Fórmula empírica (notación de Hill): C₂₅H₃₆N₄O
• Número de CAS: 1128165-88-7
• Peso molecular: 408.58
• Número MDL: MFCD19690957
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥99% (HPLC)
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: yellow
• solubilidad: DMSO: 100 mg/mL, clear, yellow
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• cadena SMILES: O=C(NCCCCCCCC)/C(C#N)=C/C1=CN(CCCN(C)C)C2=C1C=CC=C2
• InChI: 1S/C25H36N4O/c1-4-5-6-7-8-11-15-27-25(30)21(19-26)18-22-20-29(17-12-16-28(2)3)24-14-10-9-13-23(22)24/h9-10,13-14,18,20H,4-8,11-12,15-17H2,1-3H3,(H,27,30)/b21-18+
• Clave InChI: MYIMRONQBLZJFI-DYTRJAOYSA-N

Descripción

Descripción general

A cell-permeable, allosteric Dynamin inhibitor (IC₅₀ = 6.9 µM and 14 µM for full length Dynamin I and Dynamin II GTPase activity, respectively, in vitro). 10 µM of this compound results in cytokinesis failure at the abscission stage in HeLa cultures, with 82% of cells that enter into mitosis becoming multinucleated, and with 97% resulting in cell death. However, it displays a much reduced effect in NIH3T3 cells at the same concentration. It elicits a dose-dependent reduction in cell growth against SMA-560, SJ-G2, SW480, MCF-7 and A2780 cultures, as being the most sensitive among a panel of other cancer cell lines (GI₅₀ = 4.0–37 µM). It was further shown to be 6-fold more active (IC₅₀ = 5.0 µM) than dynamin mediated receptor mediated endocytosis (RME) in U2OS cells, and 15-fold more active (IC₅₀ = 1.3 µM) than Dynasore (Cat. No. 324410) in another Dynamin I GTPase activity assay.

Envase

Packaged under inert gas

Advertencia

Toxicity: Standard Handling (A)

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Chircop, M. et al. 2011. Mol. Cancer Ther.10, 1553.
Hill, T.A., et al. 2009. J. Med. Chem.52, 3762.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable