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Documentos clave

12660

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2

Sinónimos:

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.25

assay

≥96% (SDS-PAGE)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤5% Moisture

color

off-white

solubility

0.15 M sodium chloride: 10 mg/mL

cation traces

heavy metals: ≤50 ppm

shipped in

ambient

storage temp.

2-8°C

General description

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2 is a native fatty acid profile that reflects endogenous lipids. It has a pH in the range of 4.8-5.4 in 150 mM NaCl (1%).
Bovine serum albumin (BSA), a non-glycosylated globular, α-helical protein, belongs to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2 has been used as a blocking agent in western blotting. It has also been used as a component in phosphate-buffered saline (PBS) for diluting secondary antibodies and blocking in immunofluorescence to observe the localization of the Golgi apparatus.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most vital component of cell culture media. It aids in the differentiation of embryonic stem cells (hESC). It plays a key role in the transportation of drugs, hormones, and fatty acids. BSA is used in enzyme-linked immunosorbent assay (ELISA) as a blocking agent.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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