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201634

Sigma-Aldrich

6-Aminofluorescein

Sinónimos:

Fluoresceinamine isomer II

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About This Item

Fórmula empírica (notación de Hill):
C20H13NO5
Número de CAS:
51649-83-3
Peso molecular:
347.32
Beilstein/REAXYS Number:
51708
EC Number:
257-334-7
MDL number:
MFCD00005051
UNSPSC Code:
12171500
PubChem Substance ID:
24852016
NACRES:
NA.47

form

powder

mp

285 °C (dec.) (lit.)

solubility

methanol: 1 mg/mL, clear, yellow to very dark yellow-orange

λmax

495 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2

InChI key

YOAWSYSKQHLFPM-UHFFFAOYSA-N

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Application

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes′. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.
6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U).

Other Notes

Note: Do not confuse with fluorescamine.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Self assembly of amphiphilic C60 fullerene derivatives into nanoscale supramolecular structures
Partha R
Journal of Nanobiotechnology (2007)
W.E.G. Muller and H.C. Schroder
Biological Response Modifiers ? Interferons, Double-Stranded RNA and 2?,5?-Oligoadenylates (2012)
I Climent et al.
Analytical biochemistry, 182(2), 226-232 (1989-11-01)
Oxidative modification of proteins is implicated in a number of physiologic and pathologic processes. Metal-catalyzed oxidative modification usually causes inactivation of enzymes and the appearance of carbonyl groups in amino acid side chains of the protein. We describe use of
K C Ingham et al.
Biochimica et biophysica acta, 670(2), 181-189 (1981-09-29)
A method for covalent attachment of a fluorescent molecule to the carbohydrate moieties of glycoproteins is described. The glycoproteins were oxidized with periodate under mild conditions selective for sialic acid (Van Lenten, L. and Ashwell, G. (1971) J. Biol. Chem.
Miju Kim et al.
ACS nano, 9(8), 8269-8278 (2015-07-15)
Polyelectrolyte microcapsules represent versatile stimuli-responsive structures that enable the encapsulation, protection, and release of active agents. Their conventional preparation methods, however, tend to be time-consuming, yield low encapsulation efficiency, and seldom allow for the dual incorporation of hydrophilic and hydrophobic
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