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SRP3258

Sigma-Aldrich

DKK-1 human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

Synonym(s):

Dickkopf-1, Dickkopf-related protein-1, SK

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥97% (HPLC)
≥97% (SDS-PAGE)

form

lyophilized

mol wt

35-40 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

suitability

suitable for molecular biology

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DKK1(22943)

General description

DKK-1 (dickkopf WNT signaling pathway inhibitor 1) is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. Recombinant human DKK-1 expressed in human 293 cells is a 35-40kDa glycoprotein containing 235 amino-acid residues.
Research Area: Cell Signaling
The Dkk-1 gene is a prototype member of a newly identified gene family that encodes secreted glycoproteins responsible for regulating cell fate and neural patterning during embryonic development.

Application

DKK-1 human has been used for the preincubation of human adipose-derived stromal/stem cells before growing them in neuron differentiation cocktail medium. It has also been used for the differentiation of induced pluripotent stem cells into photoreceptor progenitor cells.

Biochem/physiol Actions

DKK-1 (dickkopf WNT signaling pathway inhibitor 1) was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 play an important regulatory role in the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/β-catenin signaling system. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. In bladder cancer, DKK-1 is overexpressed in serum and might be associated with tumor progression. It is also associated with multiple myeloma and affects bone formation. Changes in serum levels of DKK1 is linked with inflammation in rheumatoid arthritis. In the process of development, tumorigenesis, and inflammation, DKK1 enhances angiogenesis.
The human Dkk-1 (hDkk-1) gene, a transcriptional target of the p53 tumor suppressor, encodes a potent inhibitor of the Wnt signaling pathway and plays a role in the spatial patterning and morphogenesis of the mammalian central nervous system. As a pro-apoptotic gene, hDkk-1 suggests significant involvement in connecting the oncogenic Wnt pathway with the p53 tumor suppressor pathway.

Sequence

TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH

Physical form

Lyophilized from 1x PBS, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Functional characterization of WNT7A signaling in PC12 cells: interaction with A FZD5 x LRP6 receptor complex and modulation by Dickkopf proteins.
Caricasole A, et al.
The Journal of Biological Chemistry, 278, 37024-37031 (2003)
DKK1 expression by synovial fibroblasts in very early rheumatoid arthritis associates with lymphocyte adhesion in an in vitro flow co-culture system.
Juarez M, et al.
Arthritis Research & Therapy, 18, 14-14 (2016)
Serum Dickkopf-1 levels as a clinical and prognostic factor in patients with bladder cancer.
Sun DK, et al.
Genetics and molecular research : GMR, 14, 18181-18187 (2015)
Human Dkk-1, a gene encoding a Wnt antagonist, responds to DNA damage and its overexpression sensitizes brain tumor cells to apoptosis following alkylation damage of DNA
Shou J, et al.
Oncogene, 878-889 (2002)
Downregulation of MicroRNA-152 contributes to high expression of DKK1 in multiple myeloma.
Xu Y, et al.
RNA Biology, 12, 1314-1322 (2015)

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