E3889

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

for molecular biology, ≥97.0%

• Synonym(s): EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
• Linear Formula: [-CH₂OCH₂CH₂N(CH₂CO₂H)₂]₂
• CAS Number: 67-42-5
• Molecular Weight: 380.35
• Beilstein: 1717370
• EC Number: 200-651-2
• MDL number: MFCD00004291
• UNSPSC Code: 12352106
• PubChem Substance ID: 24894530
• NACRES: NA.31

Properties

• grade: for molecular biology
• Assay: ≥97.0%
• form: powder
• reaction suitability: reagent type: chelator
• loss: ≤1.0% loss on drying
• mp: 241 °C (dec.) (lit.)
• foreign activity: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• SMILES string: OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
• InChI key: DEFVIWRASFVYLL-UHFFFAOYSA-N

Description

Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used:

• in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions
• as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration
• as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis

A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol tetraacetic acid (EGTA) can chelate calcium (Ca²⁺) at a ratio of 1:1. It serves as an anti-coagulant when dissolved at 1 g per 100 ml of blood. EGTA can prevent the vesicular release in the nanodomain of single Ca²⁺ channels. This metal chelator can inhibit the activity of pseudolysin.

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves