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C7527

Sigma-Aldrich

Choline chloride

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

Synonym(s):

(2-Hydroxyethyl)trimethylammonium chloride

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About This Item

Linear Formula:
(CH3)3N(Cl)CH2CH2OH
CAS Number:
67-48-1
Molecular Weight:
139.62
Beilstein:
3563126
EC Number:
200-655-4
MDL number:
MFCD00011721
UNSPSC Code:
12352205
PubChem Substance ID:
24892968
NACRES:
NA.75
Pricing and availability is not currently available.

biological source

synthetic (organic)

product line

BioReagent

Assay

≥98%

form

crystals

mol wt

Mw 139.62 g/mol

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

white

pH

5.0-6.5 (25 °C, 140 g/L)

mp

302-305 °C (dec.) (lit.)

solubility

H2O: 50 mg/mL

SMILES string

[Cl-].C[N+](C)(C)CCO

InChI

1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

SGMZJAMFUVOLNK-UHFFFAOYSA-M

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General description

Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD).[1] It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.[2]

Application

Choline chloride has been used:
  • as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells[3]
  • for choline standard curve generation for quantifying phospholipase D (PLD) activity[4]
  • as a component of slice solution for the dissection of entorhinal-hippocampal brain slices[5]

Substrates

Choline acetyltransferase substrate

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF5409V
WXBF2863V
WXBF2516V
WXBF4888V
WXBF3420V

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Harvie P Portugaliza et al.
Scientific reports, 9(1), 14595-14595 (2019-10-12)
Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of
Functional calcium imaging in developing cortical networks
Dawitz J, et al.
Journal of Visualized Experiments, 119(56), e3550-e3550 (2011)
Determination of high-affinity choline uptake (HACU) and choline acetyltransferase (ChAT) activity in the same population of cultured cells
Ray B, et al.
Brain Research, 1297(11), 160-168 (2009)
Choline: an essential nutrient for public health
Zeisel SH and Da Costa KA
Nutr. Rev, 67(11), 615-623 (2009)
Apoptosis of bovine granulosa cells: Intracellular pathways and differentiation
Carou MC, et al.
Acta Histochemica, 119(5), 462-470 (2017)
View All Related Papers

Questions

1–3 of 3 Questions  

  1. Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?

    1 answer

    1. This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.

      Helpful?

  2. Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?

    1 answer

    1. This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w

      Helpful?

  3. Hi, could you please provide the product information sheet for Choline chloride.

    1 answer

    1. There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:

      Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdf

      Certificate of Origin
      https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921V

      Helpful?

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