MABF2788
Anti-SARS-CoV-2 nucleocapsid Antibody, clone 7B10-3E4
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
7B10-3E4, monoclonal
mol wt
calculated mol wt 45.63 kDa
observed mol wt ~50 kDa
species reactivity
virus
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Nucleocapsid protein in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein, but not in lysates from the wild type HEK293T cells.
Tested Applications
Western Blotting Analysis: A 1:20 dilution from a representative lot detected SARS-CoV-2 nucleocapsid protein in Whole cell lysates of HEK293T cells expressing the nucleocapsid (NC) proteins of various human coronaviruses (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
ELISA Analysis: A 1:3 dilution from a representative lot detected SARS-CoV-2 nucleocapsid protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot immunoprecipitated SARS-CoV-2 nucleocapsid protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Immunofluorescence Analysis: A 1:5 dilution from a representative lot detected SARS-CoV-2 nucleocapsid in HeLa cells transiently transfected with SARS-CoV-2 NC-P2A-GFP (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Storage and Stability
Other Notes
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recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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