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Key Documents

04-486

Sigma-Aldrich

Anti-RhoG Antibody, clone 1F3 B3 E5

clone 1F3 B3 E5, Upstate®, from mouse

Synonym(s):

ARHG, RHOG ras homolog gene family, member G (rho G), MGC125835, MGC125836

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1F3 B3 E5, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NM_001665

UniProt accession no.

P84095

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RHOG(391)

General description

RhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in it place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive. RhoG is believed to be crucial for integrin-mediated Rac activation and cell spreading. RhoG is believed to be active upstream of Rac and work independently of Rac.

Specificity

Recognizes RhoG

Immunogen

KLH synthetic peptide encompassing amino acids CQQDGVKEVFAEAVRAVLNPT (RhoG 160-180)

Application

Anti-RhoG Antibody, clone 1F3 B3 E5 is an antibody against RhoG for use in WB.

Quality

Routinely evaluated by western blot on NIH/3T3 cell lysate.

Target description

21 kDa

Physical form

Format: Purified
Purified mouse monoclonal IgG2b in storage buffer containing PBS with 0.05% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Trio-Rac1-Pak1 signalling axis drives invadopodia disassembly.
Moshfegh, Y; Bravo-Cordero, JJ; Miskolci, V; Condeelis, J; Hodgson, L
Nature Cell Biology null
Aneta Kwiatkowska et al.
Molecular cancer, 11, 65-65 (2012-09-13)
The invasion of glioblastoma cells into regions of the normal brain is a critical factor that limits current therapies for malignant astrocytomas. Previous work has identified roles for the Rho family guanine nucleotide exchange factors Trio and Vav3 in glioblastoma
Sergi Marco et al.
Nature communications, 12(1), 6829-6829 (2021-11-26)
Signals are relayed from receptor tyrosine kinases (RTKs) at the cell surface to effector systems in the cytoplasm and nucleus, and coordination of this process is important for the execution of migratory phenotypes, such as cell scattering and invasion. The
Kristin Franke et al.
The EMBO journal, 31(13), 2908-2921 (2012-05-17)
The small GTPase RhoG plays a central role in actin remodelling during diverse biological processes such as neurite outgrowth, cell migration, phagocytosis of apoptotic cells, and the invasion of pathogenic bacteria. Although it is known that RhoG stimulates neurite outgrowth

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