HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery^® HS C18 after Sample Prep using Discovery^® DSC-Si (Propionitrile Mobile Phase, ELSD Detection)

condition

6 mL hexane

SPE tube/cartridge

Discovery DSC-Si, 1 gm/6 mL (52656-U)

sample addition

0.12 g oil in 0.5 mL hexane

elution

10 mL hexane-diethyl ether 87-13 (v/v)

eluate post-treatment

vaporation to dryness, reconstituted into 2 mL acetone

column

Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)

mobile phase

propionitrile

flow rate

1.5 mL/min

pressure

1117 psi (77 bar)

column temp.

30 °C

detector

ELSD

injection

5 μL