HPLC Analysis of Pipecolic Acid Enantiomers on Astec® CHIROBIOTIC® T
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] acetonitrile; (50:50, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 220 nm
injection
1 μL
sample
5 mg/mL in methanol
Descripción
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC