HPLC Analysis of pBr322 DNA-Hae III Digest on TSKgel^® DEAE-NPR

column

TSKgel DEAE-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (with 5 mm DEAE-NPR guard column) (813075)

mobile phase

[A] 1 M sodium chloride/25 mm Tris-HCl, pH 9.0; [B] 25 mM Tris-HCl, pH 9.0

flow rate

1 mL/min

column temp.

ambient

detector

UV, 260 nm

injection

20 μL of 50 μg/mL Hae III digest of pBR322 DNA