HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis^® Express RP-Amide, Modified Gradient

column

Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 μm particles (53919-U)

mobile phase

[A] water; [B] acetonitrile

gradient

10 to 25% B in 30 min; 25 to 60% B in 15 min; held at 60% B for 5 min

flow rate

0.4 mL/min

column temp.

35 °C

detector

UV, 240 nm

injection

5 μL

sample

20 mg/L each (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile