G005604

HPLC Analysis of Herbal Supplement Containing Milk Thistle on Ascentis^® Express 2.7 μm C18

application for HPLC

Propiedades

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U); mobile phase: [A] water with 0.1% formic acid; [B] methanol; gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min; flow rate: 0.6 mL/min; pressure: 4105 psi (283 bar); column temp.: 35 °C; detector: UV, 254 nm; injection: 20 μL; sample: 20 mg/mL in water:ethanol (5:95); sonicate 15 minutes; filter 0.45 μm; dilute to water:ethanol (80:20)
• suitability: application for HPLC
• application(s): vitamins, nutraceuticals, and natural products

Descripción

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle containing herbal supplement.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analito

Taxifolin
Silychristin
Apigenin 7-glucoside
Silydianin
Quercitrin
Silibinin
Silybin B
Isosilybin A
Isosilybin B
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