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ZRB1374

Sigma-Aldrich

Anti-Glypican-1 Antibody, clone 1J4, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

GPC1

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit (recombinant)

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

1J4, monoclonal
recombinant monoclonal

Línea del producto

ZooMAb® learn more

Formulario

lyophilized

mol peso

calculated mol wt 61.68 kDa
observed mol wt ~90 kDa

reactividad de especies

human

reactividad de especies (predicha por homología)

monkey

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnicas

affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG

Nº de acceso UniProt

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... GPC1(2817)
rhesus monkey ... Gpc1(698326)

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone 1J4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glypican-1. It targets an epitope within 24 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated liner peptide corresponding to 24 amino acids from the C-terminal half of human Glypican-1, isoform 1.

Aplicación

Anti-Glypican-1, clone 1J4 ZooMAb, Cat. No. ZRB1374, a recombinant Rabbit monoclonal antibody that targets Glypican-1 and is tested in Affinity Binding, Immunofluorescence, and Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Glypican-1 in MDA-MB-231 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Glypican-1 in human pancreatic cancer tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Glypican-1 in human pancreatic cancer tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Glypican-1 peptide with a KD of 4.3 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Glypican-1 (UniProt: P35052; also known as GPC1) is encoded y the GPC1 gene (Gene ID: 2817) in human. Glypican-1 is a cell-surface glycoprotein that attaches to the plasma membrane via via glycosylphosphatidylinositol (GPI) anchors. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 531-538) that are subsequently cleaved off to produce mature protein. Glypican-1 undergoes N- and O-glycosylations. N-glycosylation is mainly of the complex type containing sialic acid whereas O-glycosylation is with heparan sulfate Heparan sulfate chains are cleaved either by heparinase or are degraded by deaminative process that uses nitric oxide released from the S-nitrosylated cysteines. Glypican-1 is shown to be essential for proper skeletal muscle differentiation. It can act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It can also sequester FGF2 in lipid rafts and blocks its binding to FGF receptors. Glypican-1 is specifically enriched on cancer-cell-derived exosomes and levels of glypican-1 positive exosomes correlate with tumor burden and the survival of pre- and post-surgical patients. It is also over-expressed in human gliomas and in pancreatic cancer cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Melo, SA et al. 2015. Nature. 523(7559):177-82; Kleeff, J., et al. 1998. J. Clin. Invest. 102(9), 1662-1673).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2


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