ZMS1119

Anti-SV40 T Antigen Antibody, clone PAb416 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Anti-XLF/NHEJ1 clone 1C16 ZooMAb^® Rabbit Monoclonal, LT, LT-AG, Large T antigen
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: PAb416, recombinant monoclonal
• descripción: recombinant, expressed in HEK 293 cells
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt 81.62 kDa; observed mol wt ~80 kDa
• purificado por: using protein G
• reactividad de especies: virus
• reactividad de especies (predicha por homología): all
• envase: antibody small pack of 25
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• secuencia del epítopo: N-terminal half
• Nº de acceso Protein ID: NC_001669
• Nº de acceso UniProt: P03070
• categoría alternativa más sostenible: Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: vaccinia virus ... SV40gp6(29031019)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone PAb416 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Large T antigen of Simian virus 40.

Inmunógeno

Purified large T-antigen of SV40

Aplicación

Quality Control Testing

Evaluated by Western Blotting in lysate from SV40 infected COS-1 cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Large T antigen in lysate from SV40 infected COS-1 cells.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SV40 Large T Antigen in COS-7 cells.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected SV40 Large T Antigen in one million COS-7 Cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Large T antigen (UniProt: P03070; also known as LT, LT-AG) is encoded by the SV40gp6 gene (Gene ID: 29031019) in Simian virus 40. Large T antigen is a key early protein that is reported to be essential for both driving viral replication and inducing cellular transformation. It plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. It displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. It is shown to participates in the modulation of cellular gene expression preceding viral DNA replication and this step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. It is also known to induce the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. It inhibits host TP

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable