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Merck

T9691

Sigma-Aldrich

Anti-phospho-TrkA (pTyr490) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

Número MDL:
Código UNSPSC:
51111800
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous glycerol solution

mol peso

antigen 140 kDa

reactividad de especies

rat, mouse, human

técnicas

immunocytochemistry: 1:100 using cultured cells
immunohistochemistry (frozen sections): 1:10
immunoprecipitation (IP): 1:250
western blot: 1:1,000 using NGF-treated PC12 cells

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

phosphorylation (pTyr490)

Información sobre el gen

human ... NTRK1(4914)
mouse ... Ntrk1(18211)
rat ... Ntrk1(59109)

Categorías relacionadas

Descripción general

TrkA belongs to the Trk proto-oncogene family and is expressed mainly in central and peripheral nervous systems. When TrkA binds to NGF, it autophosphorylates tyrosine residues like-Tyr490, Tyr674, Tyr675, Tyr751, and Tyr785 and subsequently activates many downstream effector proteins. Defects in TrkA lead to congenital insensitivity to pain with anhidrosis. Anti-phospho-TrkA (pTyr490) antibody can be used in immunocytochemistry (1:100, using cultured cells). It is also useful in identifying endogenous levels of phosphorylated Tyr490 in TrkA, TrkB and TrkC. Rabbit anti- phospho-TrkA (pTyr490) antibody is reactive in mouse, human and rat.

Inmunógeno

synthetic phosphopeptide Tyr490 corresponding to residues surrounding Tyr490 of human TrkA, conjugated to KLH. This sequence is highly conserved in TrkB and TrkC.

Aplicación

Anti-phospho-TrkA (pTyr490) antibody can be used in immunoprecipitation, immunoblotting and immunohistochemistry. It may also be used in western blotting when diluted to ratio of 1:1000 for NGF-treated PC12 cells.

Forma física

Solution in 10 mM sodium HEPES, pH 7.5, and 150 mM sodium chloride containing 100 μg/ml bovine serum albumin and 50% glycerol.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Travis J Maures et al.
Molecular endocrinology (Baltimore, Md.), 23(7), 1077-1091 (2009-04-18)
The adapter protein SH2B1 (SH2-B, PSM) is recruited to multiple ligand-activated receptor tyrosine kinases, including the receptors for nerve growth factor (NGF), insulin, and IGF-I as well as the cytokine receptor-associated Janus kinase family kinases. In this study, we examine
Y Indo et al.
Nature genetics, 13(4), 485-488 (1996-08-01)
Congenital insensitivity to pain with anhidrosis (CIPA; MIM 256800) is an autosomal-recessive disorder characterized by recurrent episodes of unexplained fever, anhidrosis (absence of sweating) and absence of reaction to noxious stimuli, self-mutilating behaviour and mental retardation. The genetic basis for

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