Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
T8787
Triton™ X-100
for molecular biology
Sinónimos:
t-Octilfenoxipolietoxietanol, Éter polietilenglicol terc-octilfenil
About This Item
Productos recomendados
biological source
synthetic (organic)
Quality Level
grade
for molecular biology
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000
average mol wt 625
aggregation number
100-155
technique(s)
protein purification: suitable
protein quantification: suitable
western blot: suitable
impurities
<1.00% water (Karl Fischer)
pH
9.7
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C
transition temp
cloud point 65 °C
pour point ~7 °C
solubility
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
density
1.06 g/mL at 25 °C (lit.)
cation traces
Fe: <5 ppm
K: <0.05%
Na: <0.1%
heavy metals: <5 ppm
HLB
13.5
application(s)
hematology
histology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key
HNLXNOZHXNSSPN-UHFFFAOYSA-N
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General description
Application
- En inmunohistoquímica para teñir las retinas montadas en plano[3]
- Junto con PBS (disolución salina tamponada con fosfato) enfriada en hielo en suspensión de células para el análisis del ADN celular y el ensayo de la anexina V[4]
- Para permeabilizar las células durante los estudios microscópicos de inmunofluorescencia[5]
- Como control positivo en el ensayo de la LDH para determinar la integridad de la membrana celular[6]
- Para estimar la actividad lipasa en el plasma posheparínico utilizando el procedimiento radioenzimático Belfrage y Vaughan modificado[7]
- Para la preparación de extracto de proteínas de la membrana externa[8]
- Como componente del tampón de extracción junto con tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 para homogenización de células pulmonares de ratón.[9]
- En el tratamiento de los cortes de tejido para marcaje con inmunofluorescencia[10]
Biochem/physiol Actions
Features and Benefits
- Ideal para aplicaciones sensibles de investigación en biología molecular
- Probado para ADNasas y ARNasas
- Detergente no desnaturalizante no iónico
- Agente humectante y emulsionante superior
- Tensioactivo muy versátil
Other Notes
Legal Information
comparable product
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
483.8 °F - closed cup
flash_point_c
251 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Certificados de análisis (COA)
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Protocolos
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Contenido relacionado
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Los métodos de lisis celular y extracción de proteínas abarcan varias técnicas, desde la solubilización de detergentes hasta la ruptura mecánica, que respaldan las necesidades de investigación.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the concentration of Triton X-1000 T8787?
1 answer-
This product is considered a neat or pure liquid at 100% active ingredient. The maximum allowable water content is 1%. This value is lot specific and reported on the Certificate of Analysis.
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Hello, I am wondering how could I find the purity of the Triton X-100 in your products. Your help and advice is appreciated. Kind regards, Soheil
1 answer-
This product is 100 % active ingredient. The maximum allowable water content is 1 % for this product.
Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8787-VAR0000267901.pdfHelpful?
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Can pure Triton X-100 be stored at and remain stable at less than 4C?
1 answer-
This product has been subjected, in the pure form, to temperatures at or near the pour point (1 °C) with no degradation. At lower temperatures, the material may become viscous and require gentle warming to ensure homogeneity. In dilute solutions, such as cell lysates or protein samples, Triton X-100 is an excellent freeze/thaw stabilizer.
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What form is this in?
1 answer-
This product is in liquid form at room temperature (melting point is 6 deg C) as listed in the "Properties" section of the product detail page.
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