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T6025

Sigma-Aldrich

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

Sinónimos:

TAE buffer

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About This Item

Número MDL:
MFCD00236357
Código UNSPSC:
41105319
ID de la sustancia en PubChem:
329826837
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

esterilidad

0.2 μm filtered

Nivel de calidad

200

Línea del producto

BioReagent

Formulario

working solution

técnicas

electrophoresis: suitable

impurezas

DNase, RNase, Protease, none detected

idoneidad

suitable for electrophoresis
suitable for gel electrophoresis (after dilution to working concentration)

aplicaciones

diagnostic assay manufacturing

cadena SMILES

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

Clave InChI

HGEVZDLYZYVYHD-UHFFFAOYSA-N

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Aplicación

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA[1] and transcribed RNA samples.[2]

Otras notas

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

Nota de preparación

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000291060
SLCM3073
SLCC3584
SLBW5974
SLBQ6542V

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An in vitro enzymatic assay to measure transcription inhibition by gallium (III) and H3 5, 10, 15-tris (pentafluorophenyl) corroles
Tang GY, et al.
Journal of Visualized Experiments, 2(97), e52355-e52355 (2015)
Monica Musiani et al.
Nature protocols, 2(10), 2502-2510 (2007-10-20)
PCR is an established technique providing rapid and highly productive amplification of specific DNA sequences. The demand for equally rapid, sensitive and objective methods to achieve detection of PCR products has led to the coupling of PCR with ELISA. PCR-ELISA

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Could you provide the concentration of product T6025 and clarify if it is considered 50X, 10X, or 1X?

    1 answer

    1. The concentration for product T6025 is 40 mM Tris acetate with 1 mM EDTA at approximately pH 8.3. This solution, prepared with 18 megohm water and 0.2 μm filtration, is considered a 1X working solution.

      Helpful?

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