Further dilution in PBS with 10% FBS is a suitable option. Storing the solution with a stabilizer is recommended. As FBS contains BSA, this would be a suitable stabilizer. Avoid repeated freeze/thaw cycles. Store in working aliquots at -20°C to -80°C.
SRP3093
IL-4 human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinónimos:
B cell stimulating factor (BSF-1), BCDF, BCGF
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
human
recombinante
expressed in E. coli
Ensayo
≥98% (HPLC)
≥98% (SDS-PAGE)
Formulario
lyophilized
potencia
≤0.2 ng/mL ED50
mol peso
14.9 kDa
envase
pkg of 20 μg
técnicas
cell culture | mammalian: suitable
impurezas
<0.1 EU/μg endotoxin, tested
color
off-white to yellow
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... IL4(3565)
Descripción general
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.
Aplicación
Interleukin-4 (IL-4) has been used as a stimulant of M2 state of microglia that is associated with wound healing and debris clearance.[1]
Acciones bioquímicas o fisiológicas
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.
Secuencia
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Forma física
Lyophilized with no additives.
Reconstitución
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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After the initial reconstitution in water, can this be diluted in PBS with 10% FBS?
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