Saltar al contenido
Merck
Todas las fotos(3)

Key Documents

SRP0300

Sigma-Aldrich

TGF-β1 Latent human

recombinant, expressed in FreeStyle™ 293-F cells, ≥98% (SDS-PAGE)

Sinónimos:

LAP, TGFb1, TGFbeta1, latency-associated peptide, transforming growth factor beta-1

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(3)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in FreeStyle™ 293-F cells

assay

≥98% (SDS-PAGE)

form

aqueous solution

mol wt

25 kDa

packaging

pkg of 5 μg

manufacturer/tradename

Invitrogen

storage condition

avoid repeated freeze/thaw cycles

NCBI accession no.

NM_000660

UniProt accession no.

Q14766

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LTBP1(4052)

General description

Human TGFβ1 (GenBank Accession No. NM_000660), amino acids 30-390 (end) with Cys33Ser mutation and N-terminal His-tag, disulfide-linked homodimers of TGFβ1 LAP and mature TGFβ1, MW= ~75kDa and 25kDa, under non-reducing conditions.
LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3.

Application

Useful for cell culture and for the study of signaling and apoptotic pathways.

Biochem/physiol Actions

LTBP1 (latent transforming growth factor β binding protein 1) is an extracellular matrix protein which also controls TGF-β (transforming growth factor β) bioavailability. LTBP1 is mainly responsible for the folding and secretion of TGF-β, thereby adding it to the extracellular matrix. It is a target of HTRA1 (high temperature requirement protein A1), a serine protease involved in cellular signaling.

Physical form

Lyophilized from a 0.2 μM filtered, aqueous solution.

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA. Stability: >6 months at –70°C (as a solid). Store reconstituted protein in aliquots at –80°C for up to 3 months. Avoid freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

NMR spectroscopic and bioinformatic analyses of the LTBP1 C-terminus reveal a highly dynamic domain organisation.
Robertson IB, et al.
PLoS ONE, 9, e87125-e87125 (2014)
Cerebral small vessel disease-related protease HtrA1 processes latent TGF-? binding protein 1 and facilitates TGF-? signaling.
Beaufort N, et al.
Proceedings of the National Academy of Sciences of the USA, 111, 16496-16501 (2014)
Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1.
Saharinen J, et al.
The Embo Journal, 15, 245-253 (1996)
A suite of novel human spermatozoal RNAs.
Ostermeier GC, et al.
Journal of Andrology, 26, 70-74 (2005)
J S Munger et al.
Kidney international, 51(5), 1376-1382 (1997-05-01)
Transforming growth factor-beta are cytokines with a wide range of biological effects. They play a pathologic role in inflammatory and fibrosing diseases such as nephrosclerosis. TGF-beta s are secreted in a latent form due to noncovalent association with latency associated
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico