SHPMLIBR
MISSION® LentiPlex® Complete Mouse Pooled shRNA Library
For Rapid, Convenient Genome-wide shRNA Screens
About This Item
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product line
MISSION®
concentration
≥5x108 VP/ml (via p24 assay)
technique(s)
capture ELISA: 5 x 108 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
Categorías relacionadas
General description
The MISSION LentiPlex Complete Mouse shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 110,000 shRNA constructs from the TRC collection targeting 21,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into fifteen subpools of approximately 5,500-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.
For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.
Other Notes
Amplification and sequencing primers are also provided for downstream hit identification.
Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.
- Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
- Comprehensive, reproducible results from pooled shRNA screens
- Statistically robust and information-rich data
Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
Protocolos
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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