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Merck

SHC003

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA

Green fluorescent protein marker to monitor transduction efficiency

Sinónimos:

MISSION® Control Vectors

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About This Item

MDL number:
UNSPSC Code:
41106609
NACRES:
NA.51

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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General description

The MISSION® TurboGFP Control Vector is an 8347 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the CMV promoter. The TurboGFP Control Vector is useful as a positive transfection/transduction control in experiments using the MISSION® shRNA library clones. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA has been used to construct GATA2 overexpression vector (oeG2), by removing the GFP cassette of this vector at the 5′ NheI and 3′ PstI cloning sites and replacing it with the full-length GATA2 transcript sequence.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Hoekstra AS
Oncotarget, 6(36), 38777-38788 (2015)
Selma Zargari et al.
BMC cancer, 20(1), 789-789 (2020-08-21)
MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have established pluripotency
Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.
Diaz B
Oncotarget, 7(32), 51569-51580 (2016)
Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2.
Shearstone JR
PLoS ONE, 11(4) (2016)
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Molecular genetics & genomic medicine, 7(7), e00746-e00746 (2019-05-16)
MEIS1 (Myeloid ecotropic viral integration site 1), as a homeobox (HOX) transcription factor, has a dual function in different types of cancer. Although numerous roles are proposed for MEIS1 in differentiation, stem cell function, gastrointestinal development and tumorigenesis, the involved

Artículos

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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