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SBR00030

Sigma-Aldrich

Short Chain Fatty Acid Kit

0.2μM filtered

Sinónimos:

Sodium acetate, Sodium propionate, and Sodium butyrate

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About This Item

Código UNSPSC:
12352211
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

100

envase

pkg of 1 kit

tipo de lípido

saturated FAs

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Short chain fatty acids (SCFAs) are saturated fatty acids with less than 6 carbons. SCFAs are produced in the gut by microbial fermentation of dietary fibers.(1) The most important and researched SCFAs are Acetate (Ac), propionate (Pr) and butyrate (Bu).(2) SCFAs are predominantly found in the colon and cross the intestinal epithelium to the blood stream. SCFAs were found to be beneficial and effective in prevention of obesity, Non-alcoholic fatty liver disease and insulin resistance.(3,4)

Aplicación

SCFAs can serve in various applications including in vitro and in vivo administration to study the effect on immune system activation(5), protect epithelial cells from LPS penetration by tight junction formation induction(6), effect insulin secretion(7) and more. In these studies, SCFAs were administrated in vitro at the following concentration range Acetate 1-10mM, propionate and butyrate 0.1-1mM and in vivo Acetate, 70 mM; propionate, 30 mM; butyrate, 20 mM.
Short-chain fatty acids (SCFAs) have diverse roles in various metabolic processes, and their measurement through metabolomics can provide valuable insights into several applications.

Acciones bioquímicas o fisiológicas

Role
  • Regulate metabolism in the gut
  • Indicators of gut health and inflammation
  • Associated with gastrointestinal diseases such as IBS and other conditions such as obesity, colonic carcinogenesis, insulin sensitivity and ischemic stroke

Características y beneficios

  • High-quality chemical, suitable for mulitple research applications
  • Commonly employed in Metabolomics and Biochemical studies

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
0000112552
0000334271
0000112552
0000334271

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Myunghoo Kim et al.
Cell host & microbe, 20(2), 202-214 (2016-08-02)
Antibody production is a metabolically demanding process that is regulated by gut microbiota, but the microbial products supporting B cell responses remain incompletely identified. We report that short-chain fatty acids (SCFAs), produced by gut microbiota as fermentation products of dietary
Hui Yan et al.
PloS one, 12(6), e0179586-e0179586 (2017-06-28)
The intestinal epithelial barrier, composed of epithelial cells, tight junction proteins and intestinal secretions, prevents passage of luminal substances and antigens through the paracellular space. Dysfunction of the intestinal barrier integrity induced by toxins and pathogens is associated with a
J H Cummings et al.
Gut, 28(10), 1221-1227 (1987-10-01)
Evidence for the occurrence of microbial breakdown of carbohydrate in the human colon has been sought by measuring short chain fatty acid (SCFA) concentrations in the contents of all regions of the large intestine and in portal, hepatic and peripheral
Emanuel E Canfora et al.
Nature reviews. Endocrinology, 15(5), 261-273 (2019-01-24)
Evidence is accumulating that the gut microbiome is involved in the aetiology of obesity and obesity-related complications such as nonalcoholic fatty liver disease (NAFLD), insulin resistance and type 2 diabetes mellitus (T2DM). The gut microbiota is able to ferment indigestible
Ara Koh et al.
Cell, 165(6), 1332-1345 (2016-06-04)
A compelling set of links between the composition of the gut microbiota, the host diet, and host physiology has emerged. Do these links reflect cause-and-effect relationships, and what might be their mechanistic basis? A growing body of work implicates microbially
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Questions

1–8 of 8 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Using this kit how many times I can perform the study

    1 answer

    1. This kit contains the following components: 100 mL of 1M Sodium acetate, 100 mL of 0.5 M Sodium propionate, 100 mL of 0.5 M Sodium butyrate, and 1 mL of a short chain fatty acid mix composed of 0.1 M Sodium Acetate, Sodium Propionate, and Sodium Butyrate. Derivation reaction mixtures for detection by LC-MS/HPLC can be prepared as discussed in Table 1 of the technical bulletin.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Sodium acetate: 100 mL/0.1mL per derivation = 1000 derivations
      Sodium propionate: 100 mL/0.2 mL per derivation = 500 derivations
      Sodium Butyrate: 100 mL/0.2 mL per derivation =500 derivations
      Short Chain Fatty Acid Mix: 1 mL/0.1 mL per derivation = 10 derivations

      Please be aware the procedure provides instructions for HPLC, LC-MS and GC-MS analysis. Sample preparation will vary based upon type of analysis.

      Helpful?

  4. Hello. I purchased this product, but it is not listed in the supplementary material, so I am contacting you. What is the solvent that dissolved these SCFAs? Thank you.

    1 answer

    1. The kit components are ready-made aqueous solutions. The concentration of each component included in this kit is as follows:
      1.0 M Sodium Acetate
      0.5 M Sodium Propionate
      0.5 M Sodium Butyrate
      Short Chain Fatty Acid mix – a 0.1 M solution of each above component, Sodium Acetate, Sodium Propionate and Sodium Butyrate, in water.
      Please see the link below to review the product Technical Bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  5. Respected supplier i would like to know what is the application of the kit? Is this a HPLC standard or is it a kit used for detection of butyrate, acetate and propionate? What will i be receiving in this kit? Can you please be more specific?

    1 answer

    1. This kit may be used for a variety of applications, including calibration of downstream detection of SCFAs by HPLC, GC-MS, or LC-MS. The kit components are ready-made aqueous solutions of 1 M Sodium acetate, 0.5 M Sodium propionate, 0.5 M Sodium butyrate, and the Short chain fatty acid mix. Each buffer component has been 0.2 µM filtered and tested for endotoxin. Please see the link below to review additional information, including example analytical data and chromatograms:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  6. mi interesserebbe sapere la concentrazione del prodotto

    1 answer

    1. The concentration and volume of each component included in this kit is as follows:
      1M Sodium Acetate - 100 mL
      0.5M Sodium Propionate - 100 mL
      0.5M Sodium Butyrate - 100 mL
      Short Chain Fatty Acid mix, a 0.1M solution of each above SCFA - 1 mL
      Please see the link below to review the product Technical Bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  7. Is this kit free short chain fatty acids? I do not want the sodium attached.

    1 answer

    1. This product includes individual solutions, as well as a mixture of Sodium Acetate, Sodium Propionate, and Sodium Butyrate. Please review the product datasheet at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  8. Hello is it possible to use this kit for measuring SCFA in fish? please let me know

    1 answer

    1. Unfortunately, there are no guidelines for using this product to measure short-chain fatty acids in a fish sample. Assuming, there is a means to extract the short-chained fatty acids from a fish sample it may be possible to use this kit. Another concern is that the sample will need to be cleaned up with a sample preparation technique before utilizing LC-MS or GC-MS. It may be possible to use this kit for this purpose, but research and method development would be necessary to determine suitability for the intended application.

      Helpful?

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