SAB4700447

Anti-c-Myc Tag Antibody

mouse monoclonal, 9E10

• Sinónimos: Anti-Cellular myelocytomatosis oncogene, Anti-c-Myc
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• Nombre del producto: Monoclonal Anti-MYC , (C-terminal) antibody produced in mouse, clone 9E10, purified immunoglobulin, buffered aqueous solution
• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 9E10, monoclonal
• Formulario: buffered aqueous solution
• reactividad de especies: fusion proteins in all species, human
• concentración: 1 mg/mL
• técnicas: flow cytometry: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_002467.4
• Nº de acceso UniProt: P01106
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MYC(4609)

Descripción

Descripción general

The cellular myelocytomatosis (c-myc) gene mapped to human chromosome 8q24, is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. c-myc is a member of MYC gene family. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that regulates the G1-S cell cycle transition.

Inmunógeno

Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc

Aplicación

Monoclonal Anti-MYC, (C-terminal) antibody produced in mouse has been used in following studies:

• western blotting analysis
• chromatin immunoprecipitation quantitative PCR (ChIP-qPCR assays
• immunofluorescence microscopy

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1-5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. Membrane permeabilization is required.

Acciones bioquímicas o fisiológicas

The cellular myelocytomatosis (c-myc) oncogene plays a vital role in cellular proliferation, differentiation, apoptosis and acts as transcriptional regulator of gene expression. c-Myc expression is essential and sufficient to assist most of the cells to enter DNA synthetic (S) phase of the cell cycle. The encoded protein plays a crucial role in vasculogenesis and angiogenesis during cancer development and progression. c-Myc interacts with its binding partner Max and activates the transcription of growth promoting genes such as cyclin D2, ornithine decarboxylase and E2F1 and it also represses the transcription of multiple genes, especially p21 and p27, by binding to the transcription initiator element (Inr) in a complex with Max and either Sp1 or Miz1. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable