SAB4200696

Anti-Chondroitin Sulfate antibody, Mouse monoclonal

clone CS-56, purified from hybridoma cell culture

• Sinónimos: CSPG, chondroitin sulfate proteoglycan
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified from hybridoma cell culture; purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: CS-56, monoclonal
• Formulario: buffered aqueous solution
• reactividad de especies: mouse, chicken, bovine, human
• envase: antibody small pack of 25 μL
• concentración: ~1 mg/mL
• técnicas: immunofluorescence: 2.5-5 μg/mL using bovine BMGE cells; immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon cancer sections and Biotin/ExtrAvidin ^®-Peroxidase staining system.
• isotipo: IgM
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CSPG4(1464)

Descripción

Descripción general

Monoclonal anti-chondroitin sulfate antibody, (mouse IgM isotype) is derived from the hybridoma CS56 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with Ventral membranes of chicken gizzard fibroblasts. This antibody recognizes chondroitin sulfate containing proteoglycans (CSPG) in bovine mammary gland epithelial (BMGE) cells and also in human, chicken and mouse fibroblasts or tissues.
Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) is encoded by the gene mapped to human chromosome 15q24.2-15q24.3. It is a growth-inhibitory chondroitin sulfate proteoglycan (CSPG) produced after spinal cord injury. It has a single glycosaminoglycan (GAG) chain. The protein belongs to the transmembrane chondroitin sulfate proteoglycans family.

Inmunógeno

Ventral membranes of chicken gizzard fibroblasts

Acciones bioquímicas o fisiológicas

Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) stimulates the activation of integrins in dorsal root ganglion neurons. It also has a role in stimulating inflammatory cytokine expression in microglia. CSPG4 plays a vital role in brain repair. Overexpression of the gene leads to developmental delay in patients with trisomy of 15q24-qter. CSPG4 serves as a potential prognostic biomarker and therapeutic target in malignant cancer.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

for continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Otras notas

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Información legal

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable