Saltar al contenido
Merck
Todas las fotos(7)

Key Documents

SAB1406364

Sigma-Aldrich

Anti-RAD51 antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

Sinónimos:

BRCC5, HRAD51, HsRad51, HsT16930, RAD51A, RECA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~37 kDa

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1 μg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAD51(5888)

General description

The protein encoded by this gene is a member of the RAD51 protein family. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) The RAD51 gene is located on human chromosome 15q15.1.

Immunogen

RAD51 (NP_002866.2, 1 a.a. ~ 339 a.a) full-length human protein.

Sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Anti-RAD51 antibody produced in mouse has been used in Western blot analysis and Immunohistochemistry.

Biochem/physiol Actions

RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein replication protein A (RPA) and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage.
RAD51 specifically repairs double stranded breaks in the DNA. Upregulation of RAD51 is observed in a number of tumors and indicates poor prognosis. Rad51 induces G0/G1 to S phase transition in cell cycle. Therefore, inhibition of RAD51 reduces the progression of cancer in cases such as cervical cancer.

Physical form

Solution in phosphate buffered saline, pH 7.4

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Optional

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Role of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damageRole of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damage.
Kiyotsugu Y and Yoshio M
Cancer Science, 95(11), 866-871 (2004)
The homologous recombination protein RAD51 is a promising therapeutic target for cervical carcinoma.
Qian C, et al.
Oncology Reports, 38(2), 767-774 (2017)
The protein PprI provides protection against radiation injury in human and mouse cells.
Yi S, et al.
Scientific Reports, 6, 26664-26664 (2016)
Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA.
Golub E I, et al.
Nucleic Acids Research, 26(23), 5388-5393 (1998)
Detection of loss of heterozygosity at RAD51, RAD52, RAD54 and BRCA1 and BRCA2 loci in breast cancer: pathological correlations.
Gonzalez R, et al.
British Journal of Cancer, 81(3), 503-509 (1999)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico