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Key Documents

S200HR

Sigma-Aldrich

Sephacryl®

200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

Sinónimos:

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Número de CAS:
65546-95-4
MDL number:
MFCD00165824
UNSPSC Code:
12352202
NACRES:
NA.56

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

1-80 kDa MW range (dextrans)
5-250 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S200HR-750ML′s updated product number is GE17-0584-01

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

related product

Referencia del producto
Descripción
Precios

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Andor J Kiss et al.
The Journal of experimental biology, 207(Pt 26), 4633-4649 (2004-12-08)
The eye lenses of the Antarctic nototheniid fishes that inhabit the perennially freezing Antarctic seawater are transparent at -2 degrees C, whereas the cold-sensitive mammalian and tropical fish lenses display cold-induced cataract at 20 degrees C and 7 degrees C
Zhili Xu et al.
Methods in enzymology, 468, 147-165 (2009-01-01)
Chemical probing is widely used as a rapid approach for assessing RNA structure, folding, and function. In this chapter, we outline procedures for handling and using chemicals commonly used to probe nucleic acids. Detailed experimental conditions and design for footprinting
Valerie Waters et al.
American journal of respiratory and critical care medicine, 183(5), 635-640 (2010-10-05)
Stenotrophomonas maltophilia is one of the more common multidrug-resistant organisms isolated from the respiratory tract of patients with cystic fibrosis (CF), but the effect of chronic S. maltophilia infection on CF lung disease is unknown. To determine the impact of
M Hamilton et al.
The Journal of biological chemistry, 276(31), 29079-29090 (2001-05-19)
Phorbol ester stimulation of the MAPK cascade is believed to be mediated through the protein kinase C (PKC)-dependent activation of Raf-1. Although several studies suggest that phorbol ester stimulation of MAPK is insensitive to dominant-negative Ras, a requirement for Ras
Alberto Cruz Rodríguez et al.
Molecules (Basel, Switzerland), 25(7) (2020-04-12)
The presence of isoforms of β-glucosidase has been reported in some grasses such as sorghum, rice and maize. This work aims to extract and characterize isoform II in β-glucosidase from S. edule. A crude extract was prepared without buffer solution
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