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Merck

R6278

Sigma-Aldrich

Anti-hnRNP-U antibody, Mouse monoclonal

clone 3G6, purified from hybridoma cell culture

Sinónimos:

Anti-Heterogeneous Nuclear Ribonucleoprotein-U

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

3G6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~120 kDa

species reactivity

human, monkey

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HNRNPU(3192)

General description

HNRNPU (heterogeneous nuclear ribonucleoprotein U) is the largest protein of the HNRNP proteins, which are one of the most abundant eukaryotic nuclear protein families. HNRNPU is an abundant nucleoplasmic phosphoprotein. It has a molecular weight of 120kDa, and is composed of 806 amino acids. It contains an acidic region of amino acids succeeded by a glutamine-rich region in its N-terminal, and its C-terminal is rich in glycine, asparagine and arginine. Its RNA-binding region lies in the 112 amino acid glycine-rich stretch in the C-terminal. It is a nuclear matrix protein, and is composed of a SAF-Box, an AT-rich chromosomal region (scaffold- or matrix-attachment region (S/MAR))-binding domain, SPRY (Spla and Ryanodine receptor) domain of unknown function, and RGG (arginine-glycine-glycine) RNA-binding domain. All these three domains are conserved.
Monoclonal Anti-hnRNP-U (mouse IgG1 isotype) is derived from the 3G6 hybridoma produced by the fusion of mouse myeloma cells (Sp2/0 cells) and splenocytes from mice BALB/c immunized with RNP′s eluted from an oligo-dT cellulose column.

Immunogen

RNP′s eluted from an oligo-dT cellulose column.

Application

Anti-hnRNP-U antibody, Mouse monoclonal has been used in immunoblotting, immunoprecipitation, immunofluorescence, immunohistochemistry and immunocytochemistry.
Monoclonal Anti-hnRNP-U antibody produced in mouse has been used for WB, IHC, flow cytometry and IP.

Biochem/physiol Actions

HNRNPU (heterogeneous nuclear ribonucleoprotein U) interacts with pre-mRNA in vivo and with both RNA and ssDNA in vitro. This protein participates in various pathways, including transcriptional regulation, telomere length control and X inactivation. It is thought to be involved in the high order organization of the nucleus, as it is capable of binding both RNA and DNA. A hypomorphic mutation of HNRNPU in mice results in early embryonic death, indicating its essential role in cell viability. It participates in various events of RNA metabolism, such as, RNA transport on a viral system, regulation of RNA stability by its binding to the 3′UTR (untranslated region) of TNFα (tumor necrosis factor), and telomere length control.
Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. In the nuclei of cells infected with Vesicular Stomatitis Virus (VSV), hnRNP-U associates with the virus leader RNA, colocalizes with the virus in the cytoplasm and also is found packaged within the purified virions.

Target description

hnRNP-U belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Visite la Librería de documentos

Imaging mRNA and protein interactions within neurons
Eliscovich C, et al.
Proceedings of the National Academy of Sciences of the USA, 114(10), E1875-E1884 (2017)
Developmental Splicing Deregulation in Leukodystrophies Related to EIF2B Mutations
Huyghe A, et al.
PLoS ONE, 7(6), e38264-e38264 (2012)
Involvement of Heterogeneous Ribonucleoprotein F in the Regulation of Cell Proliferation via the Mammalian Target of Rapamycin/S6 Kinase 2 Pathway
Goh ETH, et al.
The Journal of Biological Chemistry, 285(22), 17065-17076 (2010)
Specific Interaction of Heterogeneous Nuclear Ribonucleoprotein Particle U with the Leader RNA Sequence of Vesicular Stomatitis Virus
Gupta AK, et al.
Journal of Virology, 72(11), 8532-8540 (1998)
The matrix protein hnRNP U is required for chromosomal localization of Xist RNA.
Hasegawa Y et al
Developmental Cell, 19(3), 469-476 (2010)

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