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R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Sinónimos:

Reference dye for qPCR, ROX

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About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

Formulario

solution

Nivel de calidad

300

uso

sufficient for ≥600 reactions

concentración

100 ×

técnicas

PCR: suitable

color

red

solubilidad

water: soluble

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Aplicación

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Otras notas

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

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Referencia del producto
Descripción
Precios

Pictogramas

Exclamation markEnvironment
GHS07,GHS09

Palabra de señalización

Warning

Frases de peligro

H315,H317,H319,H410

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000360620
0000353112
0000353112
0000360620
0000259390

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Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
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Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
The majority of patients diagnosed with advanced gastrointestinal stromal tumors (GISTs) are successfully treated with a combination of surgery and tyrosine kinase inhibitors (TKIs). However, it remains challenging to monitor treatment efficacy and identify relapse early. Here, we utilized a
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
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Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
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Protocolos

SYBR® Green JumpStart™ Taq ReadyMix™

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Universal SYBR Green qPCR Protocol

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