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R3757

Sigma-Aldrich

Reactive Yellow 3−Agarose

saline suspension

Sinónimos:

C.I.13245

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About This Item

MDL number:
MFCD00165714
UNSPSC Code:
23151817
NACRES:
NA.56

form

saline suspension

extent of labeling

1-5 mg per mL

matrix

cross-linked 4% beaded agarose

capacity

≥500 units/mL binding capacity (citrate synthase)

storage temp.

2-8°C

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Application

Reactive Yellow 3-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Yellow 3-agarose has been used in immunohistochemical localization studies to show where tyramine N-(hydroxycinnamoyl)transferase (THT) polypeptides occur in opium poppy. Reactive Yellow-agarose has also been used to purify human cholesteryl ester transfer protein.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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J M Stoop et al.
Archives of biochemistry and biophysics, 298(2), 612-619 (1992-11-01)
A mannitol:mannose 1-oxidoreductase was isolated from celeriac (Apium graveolens var. rapaceum) root tips by fractionation with (NH4)2SO4, followed by chromatography on a Fractogel DEAE column and then concentration with (NH4)2SO4. This newly discovered mannitol dehydrogenase catalyzes the NAD-dependent oxidation of
M L Fonda
The Journal of biological chemistry, 267(22), 15978-15983 (1992-08-05)
Human erythrocytes rapidly convert vitamin B6 to pyridoxal-P and contain soluble phosphatase activity which dephosphorylates pyridoxal-P at a pH optimum of 6-6.5. This phosphatase was purified 51,000-fold with a yield of 39% by ammonium sulfate precipitation and chromatography on DEAE-Sepharose
Purification of hydroxycinnamoyl-CoA:tyramine hydroxycinnamoyltransferase from cell-suspension cultures of <I>Solanum tuberosum</I> L. cv. Datura.
Hohlfeld, H., et al.
Planta, 199(1), 166-168 (1996)
T Vogt et al.
Planta, 203(3), 349-361 (1997-01-01)
Uridine 5'-diphosphoglucose:betanidin 5-O- and 6-O-glucosyltransferases (5-GT and 6-GT; EC 2.4.1) catalyze the regiospecific formation of betanin (betanidin 5-O-beta-glucoside) and gomphrenin I (betanidin 6-O-beta-glucoside), respectively. Both enzymes were purified to near homogeneity from cell-suspension cultures of Dorotheanthus bellidiformis, the 5-GT by
A T Dinkova-Kostova et al.
The Journal of biological chemistry, 271(46), 29473-29482 (1996-11-15)
Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such as podophyllotoxin and enterodiol, have important roles in
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