R3380
Ribonucleoside vanadyl complexes
200 mM
Sinónimos:
vanadyl ribonucleoside complexes
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
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General description
Ribonucleoside vanadyl complexes are stable complexes formed between ribonucleotides. They are low molecular weight inhibitors of cellular ribonucleases (RNases) that are used for the isolation of RNA from cells.
Application
Ribonucleoside vanadyl complexes are used as ribonuclease inhibitors during cell lysis and mRNA purification, to protect mRNA during cDNA production, and to protect RNA during digestion of DNA by DNaseI.
Ribonucleoside vanadyl complexes has been used:
- as a component of lysis buffer for cell lines prior to Poly(U) pull down assay
- to inhibit nucleases prior to immunoprecipitation (IP) of m6A-containing RNA fragment
- as a component of hybridization buffer for human retinal slices
Biochem/physiol Actions
Ribonucleoside vanadyl complexes (RVC) elicits antimicrobial functionality by inhibiting ribosomal subunit formation and suppressing the growth of Staphylococcus aureus and Escherichia coli. It is also used as a preservative solution for tissues.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant deoxyribonucleic acid technology.
R S Puskas et al.
Biochemistry, 21(19), 4602-4608 (1982-09-14)
Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
S L Berger et al.
Biochemistry, 18(23), 5143-5149 (1979-11-13)
SIRT1 functions as a negative regulator of eukaryotic poly (A) RNA transport
Shan P, et al.
Current Biology, 27(15), 2271-2284 (2017)
Transcriptome-wide mapping of N6-methyladenosine by m6A-Seq
Dominissini D, et al.
Test, 560, 131-147 (2015)
Neuroinflammatory processes, A1 astrocyte activation, and protein aggregation in the retina of Alzheimer's Disease patients, possible biomarkers for early diagnosis.
Grimaldi A, et al.
Frontiers in Neuroscience, 13, 925-925 (2019)
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