PLA0079
Rabbit anti-PCNA Antibody, Affinity Purified
Powered by Bethyl Laboratories, Inc.
Sinónimos:
ATLD2, DNA polymerase delta auxiliary protein, PCNA, cyclin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
grade
Powered by Bethyl Laboratories, Inc.
species reactivity
human, mouse
technique(s)
flow cytometry: 0.03 μg
immunohistochemistry: 1:2,000- 1:10,000
immunoprecipitation (IP): 2- 5 μg/mg
western blot: 1:1,000- 1:10,000
accession no.
P12004
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
rabbit ... PCNA(5111)
General description
Proliferating cell nuclear antigen (PCNA), a single-copy gene, spans about 12 kb. This gene is located on human chromosome 20p13. PCNA is expressed at high levels in the thymus, bone marrow, fetal liver, and some cells of the small intestine and colon. It is located in the nucleus.
Immunogen
The epitope recognized by PLA0079 maps to a region between residues 75 and 125 of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).
Application
Rabbit anti-PCNA Antibody, Affinity Purified has been used in:
- proximity ligation assay (PLA) (1:500)
- immunoblotting (1:2000)
- flow cytometry analysis
Biochem/physiol Actions
Proliferating cell nuclear antigen (PCNA) protein functions as an auxiliary factor of polymerase δ. It acts as a cofactor to polymerase ε, polymerase β, and translesion synthesis polymerases, such as η, κ, λ, and θ. In chronic lymphoid leukemia (CLL) patients, intracellular levels of PCNA protein can be used as a marker to identify clinical behavior and survival.
Physical form
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Other Notes
PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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PCNA on the crossroad of cancer
Stoimenov I, Helleday T
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2009)
James P Wells et al.
PLoS genetics, 18(12), e1010309-e1010309 (2022-12-09)
DNA replication is a vulnerable time for genome stability maintenance. Intrinsic stressors, as well as oncogenic stress, can challenge replication by fostering conflicts with transcription and stabilizing DNA:RNA hybrids. RAD18 is an E3 ubiquitin ligase for PCNA that is involved
Qiyuan Zhou et al.
American journal of respiratory cell and molecular biology, 54(5), 728-739 (2015-10-22)
We have reported that von Hippel-Lindau protein (pVHL) expression is elevated in human and mouse fibrotic lungs and that overexpression of pVHL stimulates fibroblast proliferation. We sought to determine whether loss of pVHL in fibroblasts prevents injury and fibrosis in
Emily Yun-Chia Chang et al.
Nature communications, 10(1), 4265-4265 (2019-09-21)
Ectopic R-loop accumulation causes DNA replication stress and genome instability. To avoid these outcomes, cells possess a range of anti-R-loop mechanisms, including RNaseH that degrades the RNA moiety in R-loops. To comprehensively identify anti-R-loop mechanisms, we performed a genome-wide trigenic
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