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P3543

Sigma-Aldrich

Poly-L-lysine–FITC Labeled

mol wt 15,000-30,000

Sinónimos:

Fluorescein Labeled Poly-L-Lysine

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About This Item

Número MDL:
MFCD00165427
Código UNSPSC:
12352209
NACRES:
NA.26
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

powder

Nivel de calidad

200

mol peso

precursor poly-L-lysine • HBr 15,000-30,000
 15,000-30,000

Extensión del etiquetado

0.003-0.01 mol FITC per mol lysine monomer

color

yellow to orange

temp. de almacenamiento

−20°C

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Otras notas

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000334520
0000334507
0000321146
0000301871
0000258513

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Vitreous is a barrier in nonviral gene transfer by cationic lipids and polymers.
Pitkanen L, Ruponen M, et al.
Pharmacological Research, 20, 576-583 (2003)
Leena Pitkänen et al.
The AAPS journal, 6(3), e25-e25 (2005-03-12)
We investigated the permeation of liposomal and polymeric gene delivery systems through neural retina into retinal pigment epithelium (RPE) and determined the roles of various factors in permeation and subsequent uptake of the delivery systems by RPE. Anterior parts and
Larry J Millet et al.
Lab on a chip, 10(12), 1525-1535 (2010-04-15)
Wiring the nervous system relies on the interplay of intrinsic and extrinsic signaling molecules that control neurite extension, neuronal polarity, process maturation and experience-dependent refinement. Extrinsic signals establish and enrich neuron-neuron interactions during development. Understanding how such extrinsic cues direct
Marjo Männistö et al.
The journal of gene medicine, 7(4), 466-476 (2004-12-25)
Retinal pigment epithelium (RPE) maintains the function of photoreceptors and eyesight and is an important target for gene delivery. Since in some diseases RPE cells proliferate uncontrollably, we investigated the role of cell cycle in non-viral gene delivery into a

Artículos

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique and useful properties.

Questions

1–2 of 2 Questions  

  1. ow do you determine the peptide concentration of a small scoop in solution? I am reading the absorbance at 495nm to read the fluorophore but I don't know how to convert that Absorbance value to a meaningful mg/mL or molarity concentration without an e.c.

    1 answer

    1. The viscosity average molecular weight (Mv) is determined for each lot of this product. However, there is a distribution of molecular weights around that Mv. As a result, the molar concentration is not applicable. The typical degree of polymerization (DP) for this material is approximately 100 and the typical degree of substitution (moles of FITC per mole of lysine) is approximately 0.007 or 7 per 1000; essentially 7 of 10 Poly-L-Lysine molecules contain one FITC label. The concentration of the solution may be most accurately determined by precisely weighing the sample and precisely determining the volume, so that the sample may be reported as mg/mL. The degree of substitution and degree of polymerization are lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample of lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/p3543#product-documentation

      Helpful?

  2. How much FITC quantity or weight percentage is present per mg of the mixture? If 10 mg of the product is weighed out, what portion of the weight corresponds to the PLL?

    1 answer

    1. The FITC quantity present per mg of the mixture is 0.016 mg, which corresponds to approximately 1.6% of the weight. If 10 mg of the product is weighed out, the portion of the weight corresponding to the PLL is approximately 9.84 mg.

      Helpful?

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