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Key Documents

P2918

Sigma-Aldrich

Suero humano

from platelet poor human plasma, sterile-filtered, (mycoplasma tested, virus tested)

Sinónimos:

human plasma, human sera, human serum

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

human

sterility

sterile-filtered

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

(mycoplasma tested, virus tested)
≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

The serum is the fluid part of the blood containing plasma without fibrinogen.

Application

Human Serum has been used:
  • in the preparation of human serum samples that mimic the COVID-19 patient samples for serological antibody testing
  • as a media supplement to differentiate human induced pluripotent stem cells (hiPS) to hiPS- brain microvascular endothelial cells (BMEC)
  • for culturing transgenic Plasmodium falciparum clone
  • for assessing the electrochemical response of recombinant P. falciparum parasite L-lactate dehydrogenase (LDH) in serum samples
  • in platelet-derived growth factor (PDGF) purification assays

Human serum from platelet poor plasma (PPP) is useful for studies of the effects of platelet lysates on the growth of cells such as articular chondrocytes and human skin fibroblasts. It has also been used in platelet derived growth factor (PDGF) purification assays.

Biochem/physiol Actions

Serum serves as a supplement to basal media formulations and conventional biomedical cell culture media. It acts as a source of trace nutrients, signaling molecules, growth factors, and hormones that induce proper cell growth in vitro. Human serum from platelet-poor plasma (PPP) is useful in the studies of the effects of platelet lysates on the growth of cells such as articular chondrocytes and human skin fibroblasts.

Analysis Note

Analizada la presencia de endotoxinas

Other Notes

It is not unusual for a white precipitate to form following a freeze-thaw cycle for this human serum product.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Aver Hemben et al.
Talanta, 187, 321-329 (2018-06-02)
This paper describes the development of an affinity sensor for the detection of Plasmodium falciparum parasite lactate dehydrogenase (pLDH) as one of the biomarkers used for malaria detection. The gold sensor was functionalised with anti-pLDH after cleaning the electrode surface
Physiology, Blood Plasma null
Edward N O'Neill et al.
Comprehensive reviews in food science and food safety, 20(1), 686-709 (2020-12-17)
Innovation in cultivated meat development has been rapidly accelerating in recent years because it holds the potential to help attenuate issues facing production of dietary protein for a growing world population. There are technical obstacles still hindering large-scale commercialization of
Y C Choi et al.
Arthritis and rheumatism, 23(2), 220-224 (1980-02-01)
Human platelet lysate (PL) has a strong growth promoting action on rabbit articular chondrocytes in monolayer culture. The responsible factor is heat stable (56 degrees C, 30 minutes) and above 10,000 MW. PL (80 microgram protein/ml) reduces cell protein content
Y Umeno et al.
Journal of cell science, 94 ( Pt 3), 567-575 (1989-11-01)
We examined the proliferative behaviour in tertiary culture cells of human skin fibroblasts (HSF) as well as cells of the rat 3Y1 diploid fibroblast line placed on a plastic substratum in a nutrient-rich medium containing high concentrations of platelet-poor plasma

Artículos

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