Saltar al contenido
Merck

N9657

Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Inducible antibody produced in mouse

clone NOS-IN, ascites fluid

Sinónimos:

Anti-iNOS

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

NOS-IN, monoclonal

mol peso

antigen 130 kDa

contiene

15 mM sodium azide

reactividad de especies

mouse, rat

envase

antibody small pack of 25 μL

técnicas

microarray: suitable
western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... NOS2(4843)
mouse ... Nos2(18126)
rat ... Nos2(24599)

Descripción general

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) (mouse IgG1 isotype) is derived from the NOS-IN hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. Nitric oxide synthase (NOS) is classified into three types based on molecular mass, subcellular location, and Ca2+ dependence. Type I NOS (150-160 kDa) is found in neurons. It is also called as NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kDa), best characterized in macrophages, also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kDa) is found in endothelial cells. It is called as endothelial NOS (eNOS, or ecNOS). Type II NOS is Ca2+ -independent and is expressed in activated macrophages and some glial cells after stimulation. iNOS is also found in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts.

Especificidad

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS), antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.

Inmunógeno

synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) from mouse macrophage (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.

Aplicación

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) has been used in
  • immunofluorescence staining
  • immunogold electron microscopy
  • immunoblotting
  • immunohistochemistry
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)

Acciones bioquímicas o fisiológicas

Nitric oxide synthase (NOS) is an enzyme involved in the synthesis of nitric oxide (NO), a free radical generated under physiological conditions by virtually all mammalian cells. NO is formed from arginine by NOS which oxidizes a guanidino nitrogen of arginine, releasing NO and citrulline. NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. Various types of NOS may serve a variety of diverse biological pathways.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Los clientes también vieron

Min Min Zhu et al.
Journal of inflammation (London, England), 4, 10-10 (2007-05-08)
Since airway hyperresponsiveness (AHR) and allergic inflammatory changes are regarded as the primary manifestations of asthma, the main goals of asthma treatment are to decrease inflammation and maximize bronchodilation. These goals can be achieved with aerosol therapy. Intravenous administration of
Regulation of boar sperm functionality by the nitric oxide synthase/nitric oxide system
Staicu FD, et al.
Journal of Assisted Reproduction and Genetics, 1-16 (2019)
Involvement of inducible nitric oxide synthase (iNOS) in immune-functioning of Paphia malabarica (Chemnitz, 1782)
Gajbhiye DS and Khandeparker L
Fish & Shellfish Immunology, 84, 384-389 (2019)
Ultrastructural immunogold localization of nitric oxide synthase isoforms in rat and human eosinophils
Saluja R, et al.
Cell and Tissue Research, 340(2), 381-388 (2010)
Raymond Cespuglio et al.
PloS one, 14(4), e0215070-e0215070 (2019-04-18)
To study the anatomo-biochemical substrates of brain inflammatory processes, Wistar male rats were infected with Trypanosoma brucei brucei. With this reproducible animal model of human African trypanosomiasis, brain cells (astrocytes, microglial cells, neurons) expressing the inducible nitric oxide synthase (iNOS)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico