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Merck

N6635

Sigma-Aldrich

Nutrient Mixture F-10 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Ham’s F-10

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Aplicación

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit
  • to culture satellite cells from fish white muscle of trout
  • for culturing neonatal porcine islets (NPIs)

Cantidad

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitución

Supplement with 1.2 g/L sodium bicarbonate.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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M A Silvestre et al.
Theriogenology, 58(1), 69-76 (2002-08-17)
Vitrification (3.58 M EG and 2.82 M DMSO in PBS with 20% FCS) and rapid-freezing (0.25 M sucrose, 2.25 M EG, and 2.25 M DMSO in PBS with 20% FCS) procedures were assayed to cryopreserve rabbit tissue samples from 12-day
Amirreza Khosravi et al.
Acta histochemica, 122(8), 151632-151632 (2020-11-01)
Testicular hyperthermia can have negative effects on male fertility. Despite reported therapeutic benefits of curcumin, several factors often limit its application such as low water solubility and instable structure. Curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) were designed to solve its
Azar Afshar et al.
Reproductive sciences (Thousand Oaks, Calif.), 28(2), 371-380 (2020-08-12)
Spermatogenesis process is sensitive to heat stress because the testicular temperature is 2 to 4 °C lower than the core body temperature. The current study aimed to investigate the effects of iron oxide nanoparticles containing curcumin on spermatogenesis in mice induced
Amirhosein Hasani et al.
Life sciences, 254, 117767-117767 (2020-05-15)
Heat stress shock affects the generation of free radicals and can have a harmful effect on spermatogenesis. Photobiomodulation (PBM) is very effective in andrology for treating male infertility. This research aimed at the evaluation of the impacts of PBM on
Fernanda de Mello et al.
General and comparative endocrinology, 210, 23-29 (2014-12-03)
Members of the TGF-β superfamily are involved in numerous cell functions; however, except for myostatin, their roles in the regulation of muscle growth in fish are completely unknown. We measured tgf-β1, tgf-β2, tgf-β3, inhibin βA (inh) and follistatin (fst) gene

Artículos

Nutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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