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MP0025

Sigma-Aldrich

Venor™ GeM Mycoplasma Detection Kit, PCR-based

Sinónimos:

PCR mycoplasma detection kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

usage

 kit sufficient for 25 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

Categorías relacionadas

General description

The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Application

The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

Components

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Storage and Stability

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Legal Information

Venor is a trademark of Minerva Biolabs GmbH

Solo componentes del kit

Referencia del producto
Descripción
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Anbarasu Kannan et al.
Scientific reports, 9(1), 11632-11632 (2019-08-14)
Triple negative breast cancer (TNBC) has poor survival, exhibits rapid metastases, lacks targeted therapies and reliable prognostic markers. Here, we examined metastasis promoting role of cancer testis antigen SPANXB1 in TNBC and its utility as a therapeutic target and prognostic
Enhanced neonatal Fc receptor function improves protection against primate SHIV infection.
Ko SY, Pegu A, Rudicell RS, et al.
Nature, 514(7524), 642-645 (2014)
Yanyan Li et al.
Oncotarget, 8(63), 106311-106323 (2018-01-02)
Decades of cytotoxic and more recently immunotherapy treatments for malignant glioma have had limited success due to dynamic intra-tumoral heterogeneity. The dynamic interplay of cancer cell subpopulations has been found to be under the control of proteins in the cancer
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state
Kherdjemil Y, et al.
Nature (2016)
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Protocolos

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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